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KMID : 1007520010100050534
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Food Science and Biotechnology
2001 Volume.10 No. 5 p.534 ~ p.538
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Antitumor - active , Neutral , Medium - sized Glycan from Apple Vinegar
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Matsue, Hajime
Abe, Kaoru/Arai, Rikako/Kushibiki, Toshisada/Sasaki, Jin ichi
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Abstract
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It has long been known that the consumption of apple vinegar is beneficial to health, but the chemical composition of the physiologically active constituents is not fully known. We have studied the biological effects of apple vinegar for several years and have found constituents of apple vinegar that act as an antitumor agent against experimental mouse tumors. Apple vinegar ("Okezukuri" brand; Kanesho Co. Aomori, Japan) was evaporated and dialyzed against distilled water. Non-dialyzed Fr-A was separated into break-through and absorbed fractions (Fr-to A-F1 and Fr-A-F2) using DEAF-Sepharose. Fraction A-F1 was separated into three further fractions (Fr-A-Fla, Fr-A-Flb, and Fr-A-Flc) by gel filtration with Sephacryl S-300. The antitumor activities of these fractions against Meth-A fibrosarcoma were examined using female BALB/c mice. The resulting antitumor-active substance was obtained in the Fr-A-Flc fragment which, on the basis of NMR analysis and enzymatic treatments with β-amylase and isoamylase, was a neutral medium-sized homoglycan composed of glucose only, had a molecular weight of about 10,000, and a branched structure bearing α(1-4,6) linkages.
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